Dilution-Free Analysis from Picoliter Droplets by Nano-Electrospray Ionization Mass Spectrometry
نویسندگان
چکیده
منابع مشابه
Low-cost Microfluidic Emitters for Nano-electrospray Ionization-mass Spectrometry
We report a new microfluidic emitter for nano-electrospray ionization mass spectrometry (nanoESI-MS), made from polyimide tape. In contrast to the conventional pulled glass capillary emitters typically used for nanoESI-MS, these new polyimide emitters are simple and inexpensive to make, and are disposable after a single use. Thus, sample contamination and clogging are never a problem when analy...
متن کاملOn-demand Serial Dilution Using Quantized Nano/picoliter-scale Droplets
This paper describes a fully automated droplet-based microfluidic device for on-demand serial dilution that is capable of achieving a dilution ratio of >6000 (concentration ranges from 1 mM to 160nM) over 35 nanoliter-scale droplets. This serial diluter can be applied to high throughput and label-free kinetic assays by integrating with our previously developed on-demand droplet-based microfluid...
متن کاملQuantification of Signaling Lipids by Nano-Electrospray Ionization Tandem Mass Spectrometry (Nano-ESI MS/MS)
Lipids, such as phosphoinositides (PIPs) and diacylglycerol (DAG), are important signaling intermediates involved in cellular processes such as T cell receptor (TCR)-mediated signal transduction. Here we report identification and quantification of PIP, PIP2 and DAG from crude lipid extracts. Capitalizing on the different extraction properties of PIPs and DAGs allowed us to efficiently recover b...
متن کاملAnalysis of Oligonucleotides by HPLC-Electrospray Ionization Mass Spectrometry.
A new interface procedure has been developed that allows, for the first time, the high-efficiency analysis of synthetic oligonucleotides up to 75 bases by reversed-phase HPLC and on-line electrospray ionization mass spectrometry. For oligonucleotides up to 30 bases in length, single-base resolution can be obtained with low levels of cation adduct formation in the negative ion electrospray mass ...
متن کاملIntact skin analysis by desorption electrospray ionization mass spectrometry.
In vivo skin analysis by Desorption Electrospray Ionization was characterized on healthy human volunteers by directing pneumatically assisted electrospray directly onto their fingertips. In order to eliminate the risk of electric shock, a high ohmic resistor was built into the system. Positive ion DESI-MS analysis yields low intensity spectra, while negative ion spectra feature a number of vari...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Angewandte Chemie International Edition
سال: 2009
ISSN: 1433-7851,1521-3773
DOI: 10.1002/anie.200902501